MCQ Microbiology 37

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Early attempts at the polymerase chain reaction (PCR) used E. coli DNA polymerase. This was replaced with DNA polymerase from Thermus aquaticus ("Taq" polymerase). Which of the following is the primary advantage in using this enzyme?

A. It is cheaper than E. coli polymerase

B. Specificity is increased because nonspecific hybridization of primers does not occur

C. Use of Taq polymerase results in fewer PCR cycles

D. Use of Taq polymerase enables lower temperatures to be used

E. Upon repeated cycling, Taq polymerase becomes denatured, which causes less interference with the hybridization process


Category: Microbiology MCQs



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